35例儿童急性淋巴细胞白血病DNA含量流式细胞术分析

FLOW CYTOMETRY ANALYSIS OF 35 CASES OF CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA

对35例急性淋巴细胞白血病(ALL)患儿血液标本进行了DNA含量流式细胞术(FCM)分析。依据DNA指数(DI)分类,35例中亚二倍体5例,二倍体22例,超二倍体8例。其中亚二倍体组患儿初诊时肝脾肿大及白细胞计数显著高于其它二组。患儿完全缓解(CR)后其DI值恢复正常。依据DNA含量做细胞周期分析表明,获CR后患儿的增殖期细胞百分数(P％)较初治时有显著下降。流式细胞术标本制做简单,便于临床常规使用。

Aiming at improving therapeutic effect in childhood acute leukemia 35 cases were studied by flow cytometry (FCM). Based on the DNA index, the cases were divided into three groups, hypodiploidy group (5 cases), diploidy group(22 cases), and hyperdiploidy group (8 cases). The hypoploidy patients had a high risk of treatment failure. The patients acquiring complete remission(CR) had a significant decrease in proliferation. It concludes that the FCM has an important practical value in management of leukemia.