高效液相色谱法测定呋咱甲氢龙的含量

Determination of Furazalon by HPLC

目的 采用高效液相色谱法测定去脂舒的含量。 方法 瑞典KromasieODSC1 8流动相 :水 甲醇 (1 0∶90 ) ,流速 :1ml·min- 1 ,检测波长 :2 2 0nm ,峰面积外标法。 结果 去脂舒在 50～ 50 0 μg··ml- 1 范围内呈良好的线性关系 ,r=0 .9997(n =6) ,平均回收率为 1 0 0 1 % (n6) ,RSD =0 .32 % (n =6)。 结论 本法操作简便 ,快速 ,灵敏度高 ,结果准确 ,可靠 ,重现性好。

OBJECTIVE To establish HPLC method for the determination of Furazalon.METHODS Using ODSC 18 (4.6mm×250mm,5μm)column,the mobile phase methyl alcohol water(90∶10),At a flow rate of 1.0ml·min -1 ,wavelength of the detector was 220nm.RESULTS There is a good linear relationship within the range of 50～500μg··ml -1 (r=0.9997),the average recovery is 100.1%,RSD is 0.32%(n=6).CONCLUSION The results showed that the method is simple,senstive,rapid and accurate.