肝细胞癌患者血清IL-12水平与外周血T淋巴细胞亚群变化的研究

Study on Changes of Serum Levels of Interleukin-12 and T Lymphocyte Subsets in Patients with Hepatocellular Carcinoma

目的 :探讨肝细胞癌患者血清 IL- 12水平的变化及细胞免疫功能状态。方法 :应用 EL ISA方法和流式细胞仪技术分别检测了 60名 HCC患者和 15例良性肝病对照患者血清 IL - 12水平和外周血 T淋巴细胞亚群。结果 :HCC患者血清 IL- 12水平显著性下降 ,CD+ 3、CD+ 4细胞下降 ,CD+ 8细胞升高 ,CD+ 4/ CD+ 8比值下降。IL- 12水平和 CD+ 4/ CD+ 8比值随 HCC分期升高而降低。IL- 12水平和 CD+ 4/ CD+ 8比值有显著性正相关。结论 :HCC患者细胞免疫功能受抑制。血清IL - 12水平在评价 HCC患者的细胞免疫功能、手术效果及预后方面有重要作用。 IL - 12在减慢

Objective:To investigate pre and postoperative changes of interleukin 12(IL 12) and cellular immune function in patients with hepatocellular carcinoma(HCC).Methods:Serum levels of IL 12 and T lymphocyte subsets in peripheral blood of 60 patients with HCC and 30 controls were determined by ELISA and flow cytometric technique respectively.Results:Mean serum levels of IL 12 in patients with HCC was lower than that of controls.The number of CD + 3?CD + 4 cells in HCC patients was significantly decreased and CD + 8 cells increased.The ratio of CD + 4/CD + 8 was decreased.Moreoverr,the level of IL 12 and ratio of CD + 4/CD + 8 had a good correlation with the stage of the disease and surgical procedure.Conclusion:Serum level of IL 12 would be of value in evaluating the cellular immune state,surgical effectiveness and prognosis of patients with HCC. IL 12 may play an important role in slackening the deterioration of HCC.