麦冬多糖Md-1、Md-2化学结构的研究

Study on structural features of two neutral polysaccharides Md-1、Md-2 from Ophiopogon japonicus

目的 研究中药麦冬中的多糖化学结构。方法 采用柱层析法进行纯化 ,并用红外光谱和核磁等方法阐明 Md- 1、Md- 2的化学结构。结果 Md- 1、Md- 2的相对分子质量分别为 2 70 6 4和 4 86 5 1。Md- 1、Md- 2多糖的糖单元基本组成是葡萄糖。其中 Md- 1多糖中平均每 8个葡萄糖单元中连有 1个硫酸基 ,Md- 2多糖中平均每 6个葡萄糖单元中连有 1个硫酸基。构型均为α型 ,单糖的连接方式为 1→ 4连接。结论 Md- 1、Md- 2多糖的化学结构均为有一甲醚基取代的 α- D- (1→ 4 )的葡聚糖 。

Objective To study the chemical feature of Md 1、Md 2, polysaccharides which extracted from Ophiopogon japonicus.Methods Two constituents were isolated and purified by gel filtration chromatography, and physicochemical constants, and spectral analysis further studied their structural features. Results The mean molecular weights were estimated to be 27 064 and 48 651. Structures were composed of D glucose. Conclusion Md 1 and Md 2 polysaccharides are glucans, which were composed of D glucose units joined by α (1→4)glucosidic linkages.