重症肌无力患者外周血乙酰胆碱特异性单个核细胞IFN-γ和TGF-β的变化

A STUDY ON THE LEVELS OF INTERFERON-γ AND TRANSFORMING GROWTH FACTOR-β IN PERIPHERAL BLOOD MONONUCLEAR CELL OF PATIENTS WITH MYASTHENIA GRAVIS

目的 研究重症肌无力 (myastheniagravis,MG)外周血AChR特异性单个核细胞IFN -γ和TGF -β的分泌能力 ,探讨细胞因子IFN -γ和TGF -β在MG发病机制中的作用。方法 应用体外细胞培养和ELISA方法检测MG患者外周血单个核细胞 (PBMNC)经AChR刺激后产生IFN -γ和TGF -β的水平。结果 急性期MG患者IFN -γ和TGF -β的诱生水平高于正常对照组 (P <0 0 1 ) ,AChRAb阳性组MG患者IFN -γ的诱生水平高于AChRAb阴性组 (P <0 0 1 )。

Objective To explore the roles of the cytok ine IFN-γ and TGF-β playing in the immunal machenism of MG. Methods The levels of IFN-γ and TGF-β in peripheral blood mon o clear cells induced by acetylcholine reaptor(AChR) were measured with the extern al cell culture and enzyme linked immunosorbens assay(ELISA). Results The levels of IFN-γ and TGF-β in the peripheral blood mononuclear(P<0.01,P<0.01,respectively);the l evels of IFN-γ in the AchRAb positive MG were higher than those in the negative group(P<0.01). Conclusion Both positive and negative immunal mechanisms were a ctivated in acute MG patients.