核酸荧光定量PCR检测“疣毒净”洗剂对体外人乳头瘤病毒清除作用的实验研究

目的:观察中药“疣毒净”洗液在体外对HPV的影响,以阐明其治疗机理。方法:从临床切除的CA标本中提取HPV,加入中药“疣毒净”后,提取HPV-DNA,用FQ-PCR法测定其病毒含量。结果:含HPV的试管加入“疣毒净”后,经3次反复测试,24h即不能测出HPV,而对照管及非相关中药管均含同等浓度的HPV-DNA拷贝。结论:“疣毒净”具有明确的杀灭HPV的作用,这可能是它治疗CA的机制。

Purpose: Observation of the influence of Chinese medicine ' Verrucous Abluent ' liquid to HPV in vitro, in order to expose the curing mechanism of it .Method:After drawing HPV from the CA sample cut off in clinic and adding Chinese medicine ' Verrucous Abluent ' liquid into the HPV, draw out HPV-DNA and measure its virus content by FQ-PCR . Result:By 3 times measuring repeatedly , HPV could not be surveyed out in 24 hours in the testing tube of ' Verrucous Abluent ' liquid while contrast tube and non-correlative Chinese medicine tube both had equal concentration of HPV-DNA copy . Conclusion:' Verrucous Abluent ' liquid has exact action of killing HPV, which perhaps is its mechanism of curing CA.