麻风康复者外周血Treg细胞及IL-10水平检测

Detection of Treg Cells and IL-10 in the Peripheral Blood of the Cured Patients with Leprosy

目的:通过检测麻风康复者外周血中CD4^+CD25^+Foxp3^+Treg细胞百分比、IL-10的表达水平,探讨麻风康复者的细胞免疫水平。方法:采用流式细胞技术检测25例麻风康复者和20名健康者对照的外周血中CD4^+CD25^+Foxp3^+Treg细胞的水平变化,ELISA法检测血清中IL-10水平,并进行相关性分析。结果:麻风康复者组外周血CD4^+CD25^+Foxp3^+Treg细胞占CD4^+CD25^+Treg淋巴细胞的比率和血清IL-10浓度分别为(7.45±1.43)%和(13.45±3.61)pg/mL,均高于正常对照组的(5.89±1.21)%和(9.23±1.51)pg/mL(P<0.01)。麻风康复者及正常对照外周血CD4^+CD25^+Foxp3^+Treg细胞水平与血清中IL-10含量呈正相关(P<0.05)。结论:麻风康复者外周血中CD4^+CD25^+Foxp3^+Treg细胞与血清中IL-10高于正常对照,麻风康复者可能存在细胞免疫失调状态。

Objective:To detect the level of CD4^+CD25^+Foxp3^+Treg cells and interleulin-10(IL-10)in the peripheral blood from the cured patients with leprosy in order to explore the mimmune status of them.Methods:The levels of CD4^+CD25^+Foxp3^+Treg in T lymphocyte subgroups and IL-10 in peripheral blood were detected by flow cytometry and ELISA respectilvely in 25 cured patients with leprosy and 20 healthy controls.Results:The level CD4^+CD25^+Foxp3^+Treg in T lymphocytes and the level of IL-10 in the patients were(7.45±1.43)%and(13.45±3.61)pg/mL,which were higher than those in controls(5.89±1.21)%and(9.23±1.51)pg/mL,with significant differences(P<0.01).There was a significant positive correlation between CD4^+CD25^+Treg cells and IL-10 in the cured patients and healthy controls.Conclusion:The level of CD4^+CD25^+Foxp3^+Treg cells and IL-10 in blood serum in the cured patients with leprosy were higher than those in healthy controls,which indicated the cured patients with leprosy may have Immune imbalance.