肺表面活性物质对哮喘大鼠粘附分子的影响

Effect of pulmonary surfactant on intercellular adhesion molecule -1 in rats with allergic asthma

目的 观察吸入肺表面活性物质(Pulmonary surfactant,PS)对哮喘大鼠模型哮喘发作、血清可溶性细胞间粘附分子(sICAM-1)及支气管肺组织细胞间粘附分子ICAM-1表达的影响,探讨PS防治哮喘的机制。方法 建立哮喘大鼠模型,氧气驱动雾化吸入PS治疗,采用双抗体夹心ELISA法测定其血清中sICAM-1含量,以免疫组织化学技术检测支气管肺组织内ICAM-1的表达。结果 哮喘组血清中sICAM-1含量及支气管肺组织ICAM-1表达明显增加;治疗组血清sICAM-1含量、支气管肺组织ICAM-1表达较哮喘组明显减少。结论ICAM-1在哮喘发病机制中可能发挥重要作用,外源性PS能减轻哮喘发作,其机制可能与抑制ICAM-1合成及释放有关。

Objective To observe the effect of pulmonary surfactant (PS) on intercellular adhesion molecule-1 (ICAM-1) in asthmatic rats and discuss the mechanism of the preventive and therapeutic effect of PS on asthma. Methods The models of asthmatic rats were established, treated with inhaled PS driven by oxygen before evoking them, and their asthmatic condition and the patho -logical condition of their bronchopulmonary tissue were observed. The concentration of the soluble form of intercellular adhesion molecule-1 (sICAM-1) in serum was tested with sandwich-enzyme-linked immunosorbent assay (ELISA) and the distribution of ICAM -1 in lungs was shown with immunohistochemistry technique. Results In the asthmatic group, sICAM-1 and the distribution of ICAM-1 in lungs increased significantly than that in controls. PS can alleviate asthmatic condition and pathological variation. Meanwhile in the therapeutic group, sICAM-1 and ICAM-1 expression of their bronchopulmonary decreased significantly than that in asthmatic rats. Conclusion ICAM-1 may play an important role in the pathogenesis of asthma and exogenous PS may prevent or alleviate asthma through reducing the synthesis and release of ICAM-1.