摘要
[Objectives]To study immunoregulatory effects of Nuhuang Fuzheng Granules on mice with Cytoxan-induced immunosuppression.[Methods]Kunming variety mice were randomly divided into the control group,model group,positive medicine group,and low,medium,and high dosage groups of Nuhuang Fuzheng Granules. Except the control group,the rest groups of mice were injected with Cytoxan at60 mg/kg to reproduce the immunosuppressive mice model,and were fed through stomach at 0. 5g/kg,1. 0 g/kg and 2. 0 g/kg Nuhuang Fuzheng Granules for seven days( twice a day); the positive control group was fed with Astragalus Polysacharin( 500 mg/L). The carbon clearance index,immune organ index,serum hemolysin were determined. IL-2,IFN-γ,and IL-4 levels in mouse serum were measured by ELISA. [Results] Nuhuang Fuzheng Granules could significantly incease the carbon granular expurgation index of mice with Cytoxan-induced immunosuppression,and the 2. 0 g/kg and 1. 0 g/kg dosage groups had better effect than 0. 5 g/kg dosage group( P < 0. 05); the spleen index of mice with Cytoxan-induced immunosuppression increased significantly and there was dose-effect relationship in a certain range of concentration; the thymus index and serum hemolysin HC_(50) were significantly higher than 0. 5 g/kg dosage group( P < 0. 05); IL-4 and IFN-γ in the serum was significantly higher than the model group( P < 0. 01),the increase of IL-2 was not significantly( P > 0. 05). [Conclusions] Nuhuang Fuzheng Granules can promote growth of immune organs and secretion of cytokines through improving the carbon granular expurgation ability of mice with Cytoxan-induced immunosuppression.
[Objectives]To study immunoregulatory effects of Nuhuang Fuzheng Granules on mice with Cytoxan-induced immunosuppression.[Methods]Kunming variety mice were randomly divided into the control group,model group,positive medicine group,and low,medium,and high dosage groups of Nuhuang Fuzheng Granules. Except the control group,the rest groups of mice were injected with Cytoxan at60 mg/kg to reproduce the immunosuppressive mice model,and were fed through stomach at 0. 5g/kg,1. 0 g/kg and 2. 0 g/kg Nuhuang Fuzheng Granules for seven days( twice a day); the positive control group was fed with Astragalus Polysacharin( 500 mg/L). The carbon clearance index,immune organ index,serum hemolysin were determined. IL-2,IFN-γ,and IL-4 levels in mouse serum were measured by ELISA. [Results] Nuhuang Fuzheng Granules could significantly incease the carbon granular expurgation index of mice with Cytoxan-induced immunosuppression,and the 2. 0 g/kg and 1. 0 g/kg dosage groups had better effect than 0. 5 g/kg dosage group( P < 0. 05); the spleen index of mice with Cytoxan-induced immunosuppression increased significantly and there was dose-effect relationship in a certain range of concentration; the thymus index and serum hemolysin HC_(50) were significantly higher than 0. 5 g/kg dosage group( P < 0. 05); IL-4 and IFN-γ in the serum was significantly higher than the model group( P < 0. 01),the increase of IL-2 was not significantly( P > 0. 05). [Conclusions] Nuhuang Fuzheng Granules can promote growth of immune organs and secretion of cytokines through improving the carbon granular expurgation ability of mice with Cytoxan-induced immunosuppression.
基金
Supported by Fundamental Research Funds of Chongqing Municipality(16423)
Chongqing Agricultural Science and Technology Achievements Transformation Fund Project(cst2014jcsf-nycgzha10002)
