摘要
[Objectives] To study the chemical constituents of Pilea sinofasciata C. J. Chen. [Methods] Column chromatography,such as silica gel,Sephadex LH-20 and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS,1H NMR and^(13)C NMR and physical constants were used to elucidate their structure. [Results]Ninteen compounds were isolated from 95% ethanol extracts of Pilea sinofasciata C. J. Chen,including α-tocopherol(1),stigmasterol(2),epihernandulcin(3),hernandulcin(4),benzoic acid(5),ethyl linolenate(6),ethyl hexadecanoate(7),α-amyrin(8),palmitic acid(9),behenic acid(10),adenosine(11),indole-3-carboxylic acid(12),protocatechuate(13),gallic acid(14),betulinic acid(15),oleanolic acid(16),potassium nitrate(17),diosmetin7-O-β-D-glucopyranoside( 18),3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid(19). [Conclusions] All of the compounds were isolated from the plant for the first time.
[Objectives] To study the chemical constituents of Pilea sinofasciata C. J. Chen. [Methods] Column chromatography,such as silica gel,Sephadex LH-20 and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS,1H NMR and^(13)C NMR and physical constants were used to elucidate their structure. [Results]Ninteen compounds were isolated from 95% ethanol extracts of Pilea sinofasciata C. J. Chen,including α-tocopherol(1),stigmasterol(2),epihernandulcin(3),hernandulcin(4),benzoic acid(5),ethyl linolenate(6),ethyl hexadecanoate(7),α-amyrin(8),palmitic acid(9),behenic acid(10),adenosine(11),indole-3-carboxylic acid(12),protocatechuate(13),gallic acid(14),betulinic acid(15),oleanolic acid(16),potassium nitrate(17),diosmetin7-O-β-D-glucopyranoside( 18),3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid(19). [Conclusions] All of the compounds were isolated from the plant for the first time.
基金
Supported by Innovative Common Key Technology Research and Demonstration Project on Zhuang Medicine(Gui Ke Zhong 1355001-4,14124002-11)
Guangxi Natural Science Foundation(2014GXNSFBA118142)
Independent Research Project of Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards(Gui Zhong Zhong Zi 201408)
Open Research Project of Guangxi Key laboratory of Chinese Medicine Efficacy Research(14-045-12-K3)
