摘要
用全细胞膜片钳、Western印迹法和逆转录 PCR技术 ,观察蛋白激酶C ( proteinkinaseC ,PKC)对大鼠支气管平滑肌细胞 (bronchialsmoothmusclecells,BSMCs)电压依赖性延迟整流钾通道 (KV)活性及其亚型KV1 5表达的影响。结果为 :( 1)PKC激活剂豆蔻酰佛波醇乙酯 (phorbol 12 myristate 13 acetate ,PMA)显著抑制急性分离大鼠BSMCs的KV 通道电流 ,该效应被PKC阻断剂Ro3 1 82 2 0显著抑制 ;( 2 )PMA显著抑制体外培养大鼠BSMCs的KV1 5mRNA和蛋白质的表达 ,该效应被Ro3 1 82 2 0显著抑制。上述观察结果提示 ,PKC活化可抑制大鼠BSMCs的KV 通道电流活性 ,下调KV1
The effect of protein kinase C (PKC) signaling pathway on the activity of voltage-dependent delayed rectifier potassium channel (K V) and the expression of K V isoform K V1 5 in rat bronchial smooth cells (BSMCs) were investigated with whole-cell patch clamp, Western-blot and RT-PCR techniques. The results showed: (1) phorbol 12-myristate 13-acetate (PMA), a PKC activator, caused a significant inhibition of K V channel currents in rat BSMCs. The inhibition was partly abolished by Ro31-8220, a PKC inhibitor. (2) PMA caused a significant suppression of the expression of K V1 5 mRNA and protein in rat BSMCs. These effects were attenuated by Ro31-8220. The results suggest that in rat BSMCs PKC activation inhibits K V currents and down-regulates the expression of K V1 5.
出处
《生理学报》
CAS
CSCD
北大核心
2003年第2期135-141,共7页
Acta Physiologica Sinica
基金
ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (No 30 2 70 5 83)
关键词
支气管
平滑肌细胞
电压依赖性延迟整流钾通道
蛋白激酶C
bronchus
smooth muscle cells
voltage-dependent delayed rectifier potassium channel
protein kinase C
