尼氟灭酸对肝癌细胞增殖的影响

Effects of niflumic acid on the proliferation of human hepatoma cells

为了观察氯通道阻断剂尼氟灭酸 (NFA)对人肝癌细胞 (humanhepatomacellline ,HHCC)增殖的影响 ,我们将NFA作用于HHCC ,应用细胞计数法及噻唑兰 (MTT)比色分析法观察细胞增殖情况 ;用流式细胞仪检测细胞周期时相 ;并用激光扫描共聚焦显微镜检测 [Ca2 +]i的变化。结果发现 ,NFA使HHCC细胞数及MTT光吸收值(OD)较对照组都显著降低 ,去除NFA后 ,OD值逐渐恢复。经 10 0 μmol/LNFA处理 48h的HHCC细胞G1期细胞比例比对照组明显增高 ,S期及G2 期细胞比例明显低于对照组。细胞外应用NFA ( 10 0 μmol/L)使 [Ca2 +]i快速降低 ,去除NFA后 ,[Ca2 +]i 可恢复。这些结果表明 ,尼氟灭酸能抑制细胞增殖 ,其机制可能与细胞内信号转导Ca2

The purpose of this work was to investigate the effects of niflumic acid (NFA), a chloride channel blocker, on the proliferation of human hepatoma cell line (HHCC). Cell proliferation was analyzed by cell count and MTT assay. Cell cycle analysis was carried out by flow cytometry. i was determined by laser scanning confocal system. It was found that NFA decreased significantly the cell number and the MTT optical density (OD) of HHCC cells, and that the OD value was reversed after washout of NFA. Compared with control, NFA blocked cell cycle progression in G 1 phase. Extracellular application of NFA (100 μmol/L) induced a rapid decrease in i. These findings demonstrate that blockage of chloride channels by NFA induces growth arrest of HHCC in G 1 phase, which may be due to the inhibition of Ca 2+/CaM-dependent signaling pathways.