肿瘤坏死因子-α对大鼠中脑神经干细胞分化和寡突胶质细胞增殖的影响

Effect of tumor necrosis factor-α on differentiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat

为观察肿瘤坏死因子对神经干细胞 (NSCs)分化的影响 ,本研究应用体外扩增的新生大鼠中脑NSCs,使用免疫组织化学技术 ,观察了肿瘤坏死因子 α (TNF α )对NSCs分化及其后代细胞的影响。结果显示 :( 1)TNF α可提高中脑NSCs后代中神经元和寡突胶质细胞所占的比例 ;( 2 )TNF α可明显诱导由NSCs分化的寡突胶质细胞增殖 ,但对星形胶质细胞的增殖作用不明显。上述观察结果提示TNF

To observe the influence of tumor necrosis factor-α (TNF-α) on differentiation of rat mesencephalic neural stem cells (NSCs), the numbers of neurons, astrocytes and oligodendrocytes generated from NSCs were analyzed after differentiation for 3 days by using immunocytochemistry technique. The results show that: (1) TNF-α enhanced the proportions of neurons and oligodendrocytes in progeny of NSCs; and (2) TNF-α induced the proliferation of oligodendrocytes derived from NSCs, but the proliferation of astrocytes was not influenced by TNF-α. We conclude that the TNF-α could influence the application of NSCs.