阴囊升温对大鼠睾丸生精细胞凋亡及增殖细胞核抗原表达的影响

Significance of hyperthermic treatment on apoptosis and proliferating cell nuclear antigen expression in rat germ cell

目的 研究阴囊升温对大鼠睾丸生精细胞凋亡及增殖细胞核抗原 ( PCNA)表达的影响 ,探讨其在升温引起生精细胞变化中的意义。 方法 应用原位末端标记( TUNEL )法和免疫组化法 ,检测大鼠睾丸局部升温至 43℃、3 0 min后 ,经 0 .5、1、3、6、1 0和 50 d观察生精细胞凋亡和 PCNA表达情况。 结果 大鼠睾丸局部升温至 43℃、3 0min后生精上皮受到损伤 ,生精细胞凋亡增加 ( P<0 .0 1 ) ,生精细胞 PCNA表达下降( 0 .5～ 1 0 d组 P<0 .0 1 ,50 d组 P<0 .0 5)。 结论 43℃、3 0 min作用后生精细胞 PCNA表达与细胞凋亡呈负相关 。

Objective: To explore the significance of hyperthermic treatment on apoptosis and proliferating cell nuclear antigen (PCNA) expression in rat germ cell. Methods: Germ cell apoptosis and PCNA expression were detected on day 0.5,1,3,6,10 and 50 after heat exposure (43℃ 30 min) by means of TdT mediated dUDP X nick end labeling(TUNEL) and immunohistochemistry. Results: After hyperthermic treatment, seminiferous epithelium was damaged. Germ cell apoptosis was increased ( P<0.01 ) and PCNA expression was decreased( 0.5~ 10 d: P<0.01 ;50 d: P<0.05 ). Conclusion: There was a negative correlation betweeen PCNA expression and apoptosis in rat germ cell after hyperthermic treatment. The combined determination plays an important role in the evaluation of hyperthermic treatment on spermatogenesis.