大鼠血清表皮生长因子和睾丸表皮生长因子受体与精子生成的相关性

The correlation between serum epidermal growth factor/testicular epidermal growth factor receptor and spermatogenesis in rat

目的 探讨血清表皮生长因子 ( EGF)和睾丸组织表皮生长因子受体 ( EGF-R)与大鼠精子生成的关系。 方法 40只性成熟期雄性 SD大鼠 ,随机分为假手术组( SOG)、去颌下腺组 ( SG)、去颌下腺加腹腔注射 EGF I组 ( SG-EGF I)和 II组 ( SG-EGFII) ,每组 1 0只。SG-EGF I和 SG-EGF II分别腹腔内注射 EGF0 .2 5和 0 .50 μg·kg- 1·d- 1。大鼠常规喂养 48d,断头取血和睾丸。放射免疫法检测血清 EGF水平 ,病理检查睾丸生精功能和免疫组织化学检测睾丸组织 EGF-R的表达。 结果 大鼠血清 EGF水平SG-EGF I组明显下降 ( P<0 .0 5) ,SG组有非常显著下降 ( P<0 .0 1 ) ;睾丸生精功能中、重度障碍 ;间质细胞 EGF-R表达明显减少 ( P<0 .0 5)。补充不同剂量的 EGF对睾丸生精功能有不同影响。 SOG、SG-EGF I和 SG-EGF II大鼠睾丸生精细胞、支持细胞及间质细胞 EGF-R表达无显著性差异 ( P>0 .0 5)。 结论 EGF对精子发生具重要的调控作用 ,血清 EGF水平和睾丸间质细胞

Objective: To investigate the correlation between epidermal growth factor (EGF)/testicular epidermal growth factor receptor (EGF R) and spermatogenesis in rat. Methods: Forty mature male Spraque Dawley (SD) rats were randomly assigned to one of four groups:sham operation group (SOG),sialoadenectomy group (SG),sialoadenectomy group with injection of EGF 0.25 μg·kg -1 ·d -1 (SG EGF I)and sialoadenectomy group with injection of EGF 0.50 μg·kg -1 ·d -1 (SG EGF II). Ten rats were for each group. The experimental rats were routinely feed,and the blood and testes were obtained on day 48 after operation. Serum EGF concentrations were determined by radioimmunoassay (RIA), expression of EGF R in testes examined by immunohistochemical method, and the spermatogenesis was pathologically checked. Results: Serum EGF concentrations in SG EGF I and SG decreased significantly as compared with these of SOG ( P<0.05 and P<0.01 ,respectively). The testicular function of spermatogenesis showed a moderate severe impairment in SG. The expression of EGF R in Leydig cells decreased in SG ( P<0.05 ).The different dosages of EGF replacement had affected differently. There was no significant difference of EGF R in testicular germ cells, Sertoli cells and Leydig cells in SOG, SG EGF I and SG EGF II( P>0.05 ). Conclusion: EGF play an important role in the regulation of spermatogenesis. Serum EGF concentration and high expression of EGF R in testicular Leydig cells has a positive correlation with spermatogenesis.