异位子宫内膜细胞增殖及增殖动力学研究

The proliferation process and proliferation dynamics of ectopic endometrial cells

目的 探讨异位内膜细胞的增殖特性及增殖动力学。 方法 对 1 0例异位内膜组织 (试验组 )及 1 0例正常内膜组织 (对照组 )进行原代培养 ,采用免疫细胞化学检测两组内膜 ki-67表达 ,采用流式细胞术检测两组细胞的细胞周期。 结果 ( 1 )异位内膜腺上皮细胞的 ki-67积分 ( 4.2 0± 0 .55)与正常内膜腺上皮细胞 ( 3 .90± 0 .70 )相当 ( P>0 .0 5)。异位内膜间质细胞 ki-67积分 ( 2 .92± 0 .58)与正常内膜间质细胞 ( 2 .60± 0 .3 7)相当 ( P>0 .0 5)。( 2 )异位内膜 G0 / G1、G2 / M、S期的比例分别为 ( 78.1 4± 3 .51 ) %、( 1 1 .53±2 .1 8) %、( 1 0 .3 2± 1 .72 ) %与正常内膜 ( 79.2 7± 2 .49) %、( 1 1 .53± 1 .43 ) %、( 9.2 0±1 .66) %相似 ( P>0 .0 5)。 结论 ( 1 )异位内膜细胞并非高度增殖 ;( 2 )

Objective: To investigate the cell proliferative characteristics and proliferation dynamics of ectopic endometrium. Methods: The ki 67 expression and the phases of cell cycle were studied in primary cultures of ectopic endometrial cells (from the 10 women with endometrilosis) and eutopic endometrial cells (from 10 normal women without endometriosis). The phases of cell cycle were detected by flowcytometry, while the ki 67 expression was examined by immunocytochemistry. Results: (1) The ki 67 expression in both ectopic glandular and ectopic stromal cells (4.20±0.55 and 2.92±0.58, respectively) did not show significant difference from their eutopic counterparts (3.90±0.70 and 2.60±0.37, respectively)( P> 0.05 ). (2) The distributions of G 0/G 1, G 2/M,S phases in ectopic endometrial cells (78.14% ±3.51%,11.53%±2.18%,10.32%±1.72%) were roughly similar to those in eutopic endometrial cells (79.27%±2.49%, 11.53%±1.43%, 9.20%±1.66%) ( P> 0.05 ). Conclusion: (1) The ectopic endometrial cells are not hyperproliferative. (2) The cell cycle of ectopic endometrial cells is probably under the normal control.