摘要
目的 :建立测定复方西洋参胶囊中人参皂甙 Rb1 含量的方法。方法 :以乙腈 -甲醇 -水 ( 70 :3 0 :15 )为流动相 ,采用高效液相色谱法测定。结果 :人参皂甙 Rb1 在 0 .0 3～ 0 .15 mg/ ml范围内线性良好 ,回收率为 97.8% ,RSD =1.0 %。样品采用 Sep- pak C1 8柱的固相萃取技术。结论 :方法快速、简便 。
Objective: To establish a method for the determination of ginsenoside Rb1 in compound panax quinguefolium L. capsule. Methods: A simple HPLC method for determination of ginsenoside Rb 1 was established. Acetonitrile-methanol-water(70∶30∶15) was used as mobile phase. Results: The standard curve was linear within the range of 0.03~0. 15 mg/ml for ginsenoside Rb 1, the recovery was 97.8%, RSD was 1.0%. Solid phase extraction method of Sep-pak C 18 was used for samples. Conclusion: The method was quick, simple and accurate.
出处
《天津药学》
2003年第2期19-21,共3页
Tianjin Pharmacy
