幼年型类风湿关节炎患儿外周血中Th_1与Th_2比例失衡探讨

Imbalance of Th_1/Th_2 in peripheral blood patients of juvenile rheumatoid arthritis

目的:探讨JRA患儿外周血中T辅助细胞(Th_1/Th_2)分布状态。方法:应用三色荧光标记法流式细胞仪检测25例JRA患儿外周血中Th_1和Th_2的百分率,以8位择期小手术儿童作对照。结果:显示JRA患儿外周血中Th_2细胞较正常人少,但Th_1数目则与正常人无异。结论:Th_2细胞减少,可能是JRA致病原理之一。

Objective:To observe T helper cells (Th1/Th2) in peripheral blood in the patients with juvenile rheumatoid arthritis(JRA). Methods: Peripheral blood was obtained from 25 children with JRA. 8 children undergoing small operation were recruited as controls. The ratio of Th1 to Th2cells from peripheral blood mononuclear cells (PBMC) was evaluated by using intracellular staining analysis of cytokine production by 3-colored flow cytometry. Results: In comparison with normal controls,the patients with JRA had significantly fewer Th2 cells among their PBMC. The percentage of Th1 cells among PBMC was not different between patients and normal controls. Conclusion: Decreased Th2 cells might be a predominant factor in JRA pathogenesis.