IL-2对精子顶体酶及一氧化氮合酶活性的影响

Effect of interleukin-2 on the activity of acrosin and NOS of human being's sperm

目的 探讨白细胞介素 2对正常人及白细胞精子症精子功能的影响。方法 精子顶体酶的活性检测采用BAEE/ADH联合法。结果 白细胞精子症及正常人精子顶体酶的活性分别为 (2 7.6 8± 10 .0 6mU/ml,n =18;4 3.6 3± 7.6 1mU/ml,n =18) (P <0 .0 1) ;白细胞精子症及正常人精子一氧化氮合酶分别为 (2 .74± 0 .39U/mg pr,n =18;2 .74± 0 .39U/mgpr,n =18) (P <0 .0 1)。结论 白细胞介素 2对精子顶体酶和一氧化氮合酶均有明显抑制作用 ,提示这可能是导致精子受精能力下降的原因。

Objective To study the effect of interleukin 2 on the function of sperm of leukocytospermia.Methods Measuring the activity of acrosin by BAEE/ADH.Results The acrosin activity of leukocytospermia and normal sperms(27.68±10.06 mU/ml, n =18;43.63±7.61 mU/ml, n =18)( P <0.01);the nitric oxide synthase(NOS) of leukocytospermia and normal sperms(2.74±0.39 U/mgpr, n =18;2.74±0.39 U/mgpr, n =18)( P <0.01).Conclusions Interleukin 2 inhibited significantly the activity of acrosin and NOS in the human sperm,which might be one of the reasons that decrease fertilization capacity of sperm.