自发性高血压大鼠阴茎海绵体组织nNOS变化的实验研究

The alterations of nNOS-containing nerve firbers in corpus cavernosum of spontaneously hypertensive rats

目的 研究高血压对大鼠阴茎海绵体组织nNOS神经纤维的影响。方法 自发性高血压大鼠 (SHR)和同系WKY鼠各 12只 ,都随机分为 4周观察组 (6只 )和 12周观察组 (6只 )。第 4周和第 12周末时 ,观察阴茎勃起功能 ,并检测阴茎组织nNOS神经纤维数目。结果 4周组和 12周组中 ,SHR和WKY鼠在阴茎勃起次数和阴茎组织nNOS神经纤维数目上 ,都有显著差异 (P <0 .0 1)。SHR 4周组和SHR 12周组 ,在阴茎组织nNOS神经纤维数目上 ,差异也有显著性 (P <0 .0 5 ) ,阴茎勃起次数和勃起率差异无显著性 (P >0 .0 5 ) ,但有减少的趋势。结论 高血压使阴茎组织中nNOS神经纤维数目减少 ,这可能是高血压引起勃起功能障碍的发病机理之一。

Objective To investigate the effect of hypertension on nNOS containing nerve fibers in corpus cavernosum of spontaneously hypertensive rats(SHR).Methods Twenty four six weeks old male SHR and WKY rats were divided into four weeks observation group and twelve weeks observation group respectively.Each group was sacrificed for detection of nNOS containing nerve fibers after erectile function test at planned time.Results Significant difference exists in erection frequency and the number of nNOS contatining nerve fibers between SHR and WKY controls( P <0.01),and it was also of significant difference in the number of nNOS containing nerve fibers between two SHR groups( P <0.05).Conclusions The erectile function and number of nNOS containing nerve fibers in SHR were dramatically affected by uncontrolled hypertension.The decreasing of nNOS containing nerve fibers may be one of the mechanisms underlying erectile dysfunction in hypertensive subjects.