精浆蛋白电泳带谱分析对梗阻性无精子症的定位诊断价值

Electrophoregram analysis of seminal protein in emplacement diagnosis of obstructive azoospermia

目的 评估精浆蛋白电泳带谱对梗阻性无精子症的定位诊断价值。方法 对 2 8例正常生育男子和 2 1例梗阻性无精子症患者 (包括 14例双侧输精管结扎和 7例双侧附睾尾梗阻 ) ,采用不连续SDS PAGE分析精浆蛋白带谱变化 ,并分别测定精浆总蛋白质含量。结果 正常生育男子与梗阻性无精子症者比较 ,虽然总蛋白质含量有差异 (P <0 .0 5 ) ,但精浆蛋白带谱分析基本相同。结论 精浆蛋白电泳带谱分析对梗阻性无精症的诊断及定位无临床意义。

Objective To evaluate the value of seminal protein electrophoregrams in emplacemental diagnosis of obstructive azoospermia.Methods Seminal protein obtained from 28 men with normal semen analysis (control group) and 21 cases with obstructive azoopermia including 14 cases of vasectomy and 7 cases of bilateral obstruction in epididymal caput were evaluated by discontinuity SDS PAGE,and concentrations of the total protein were measured.Results The seminal protein electrophoregrams were closely similar between the control group and the azoospermia group.The total protein concentrations of the control group and the azoospermia group were 28.69±7.04 g/L, 23.40 ±6.15 g/L respectively( P<0.05 ).Conclusions There was of no clinical value for seminal protein electrophoregrams analysis in the emplacement diagnosis of obstructive azoospermia.