白细胞介素-1β和肿瘤坏死因子α在脑梗塞发病中的作用

The study of effect of IL-1β,TNF-α on cerebral infarction

目的:探讨白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)在脑梗塞(CI)发病中的作用.方法:应用PCR方法对纤溶酶原激活物抑制剂-1(PAI-1)基因启动子区-675位4G/5G基因进行检测,应用放射免疫法测定II-1β、TNF-α血浆水平,应用RT-PCR法检测其mRNA表达水平,应用发色底物法检测血浆PAI-1活性.结果:①CI患者表现为4G/4G型11例,4G/5G型30例,5G/5G型13例;②血浆PAI-1活性为(0.99±0.08)×10 3AU/I,明显高于正常对照组的(0.72±0.13)×103AU/L,差异有极显著性意义(P＜0.01);③CI患者IL-1β血浆水平为(0.45±0.24)μg/L,TNF-α血浆水平为(1.52±0.31)μg/L,正常对照组分别为(0.22±0.11)μg/L和(0.61±0.22)μg/L(P＜0.01);CI组IL-1β mRNA表达水平为0.42±0.27,TNF-α为0.82±0.65,明显高于正常对照组的0.29±0.23及0.26±0.23,经统计学处理差异有极显著性意义(均P＜0.01).结论:①IL-1β、TNF-α表达水平、血浆PAI-1活性在CI患者中明显增高,提示IL-1β、TNF-α、血浆PAI-1活性在CI发病中起重要作用.②未发现II-1β、TNF-α与PAI-1基因启动子区4G/5G基因多态性、血浆PAI-1活性有相关性.

Objective:To explore the role of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in the pathophysiology of cerebral infarction(CI). Method:The plasminogen activator inhibitorl (PAI-1)4G/5G genotype was evaluated by PCR,The serum levels of IL-1β and TNF-α were measured using radioimmunoassay; the mRNA expression of cytokines above-mentioned was detected using RT-PCR; the activity of PAI-1 was determined by chromophorous substrate method. Result:①The PAI-1 activity in patients with CI and normal controls was (0. 99 ± 0. 08)×103AU/L and (0. 72 ± 0. 13)× 103AU/L respectively( P <0 01).②The serum levels of IL-1β in CI group and normal controls was (0. 45 ±0. 24)μg/L and (0. 22± 0. 11)μg/L respectively( P <0. 01).③The serum levels of TNF-α in patients with CI and normal controls was (1. 52 ± 0. 31)μg/L and (0. 61± 0. 22)μg/L respec-tively( P <0. 01). ④The mRNA expression levels of IL-1β and TNF-α were significantly higher in CI group than those in normal controls( P <0. 01 and P <0. 01,respectively). Conclusion:①There are elevated expression of IL-1β,TNF-α and PAI-1 activity in CI patients compared with normal controls, indicating they play important roles in the pathophysiology of CI. ②There are no relationships among IL-1β,TNF-α and PAI-1 activity. 4G/5G polymorphism.