临床沙眼衣原体分离培养与鉴定方法的比较

Culture and Identification of Chlamydia Trachomatis from Clinic Specimens

目的 探讨从临床病人中 ,分离培养沙眼衣原体的标本采集、去杂菌污染 ,比较接种及沙眼衣原体鉴定的方法。方法 随机取 10 0例门诊女性标本 ,在McCoy细胞中进行分离培养沙眼衣原体 ,比较新鲜标本及运送培养基中 4℃过夜后去杂菌的差别 ;比较离心法与聚已二醇法接种对分离的影响 ;比较染色和膜免疫层析法对沙眼衣原体的鉴定。结果 分离到沙眼衣原体 9株。标本经运送培养基中 4℃过夜后杂菌污染下降 2倍 ;离心法及聚已二醇法接种沙眼衣原体的分离率无明显差异 ;膜免疫层析法对沙眼衣原体的鉴定较染色法简便 ,快速。结论 标本应4℃过夜后接种以降低污染率 ;较离心法简便的聚已二醇法可用于沙眼衣原体的培养及传代 ;膜免疫层析法对沙眼衣原体的鉴定快速方便 ,但成本较高。

Objective To study the method of specimens collection, and antimicrobial contaiminnant and to compare the assay of inoculation and identify of C trachomatis.Methods 100 specimens from the outpatients chosen randomly were cultured in McCoy cell. The differences of the inoculation of the samples handled at 4℃ overnight and directly were studied and the influence of the treatment of the samples in the centrifugation and PEG was compared. The identification of Ct was also compared with the routine stains and immunochromatographic assay.Results 9 strains of Ct were separated. The fungal contaiminnant in culture decreased to 1/2 after keeping specimen at 4℃ overnight. There was no difference in the separated rates between centrifugation and PEG. Immunochromatographic assay to recognize Ct was more convenient and quicker than traditional strain.Conclusion The sample of inoculation of Ct should be treated at 4℃ overnight for antimicrobial contaiminnant. The PEG handling is better than the centrifugation at the separated and culture passage of Ct. The immunochromatographic assay to identify the Ct is more convenient and quicker than stain assay, but a little bit costly.