沉淀-漫反射光谱法测定还原糖

Determination of reducing sugar by precipitation-reflectance spectroscopy

通过测定还原糖与斐林试剂反应生成的Cu2 O ,建立测定还原糖的沉淀 -漫反射光谱分析方法 .该方法具有操作方便 ,灵敏度高 ,试剂用量少 (<1mL)等优点 .还原糖在 2 0～ 12 0 μg范围有良好的线性关系 ,用于实际样品测定 。

Based on the reaction between Fehling reagents and reducing sugar, the diffuse reflectance spectroscopy for determining reducing sugar was established. The advantages of this method are the simplicity of operation, the sensitivity of determination and the small amount of regents required (<1 mL). The amount of reducing sugar in the range of 20～120 μg shows a good linear relationship. Using the method to determine some samples, the results are satisfied.