维生素D受体基因多态性与肺结核易感性的病例对照研究

A case-control study on the vitamin D receptor gene polymorphisms and susceptibility to pulmonary tuberclosis

目的 探讨维生素D受体 (VDR)基因多态性与中国汉族人群肺结核发病间的关系。方法 采用病例对照研究设计 ,用聚合酶链反应 限制性片段长度多态性分析 (PCR RFLP)检测VDR基因中T/C多态性位点 ,对肺结核相关的环境因素进行问卷调查 ,并进行单因素分析和多因素logistic回归分析。结果 对 76例病例和 171名对照进行了VDR的基因分型 ,VDR FF、VDR Ff与VDR ff三种基因型在病例组和对照组中的分布频率分别为 38.2 %、 4 4 .7%、 17.1%和 5 2 .6 %、4 0 .9%、6 .4 % ,其中ff基因型在病例组中的频率显著高于对照组 ,OR值 95 %CI:3.6 6 8(1.4 83～9.0 71) ;在多因素分析中调整卡介苗接种史和吸烟状况后 ,VDR ff基因型与肺结核发病仍有显著性关联 ,调整OR值 95 %CI:3.0 36 (1.117～ 8.2 5 3)。结论 VDR ff基因型可能是中国汉族人群肺结核病的易感基因型。

Objective To investigate the association between the genetic polymorphisms of VDR gene and susceptibility to pulmonary tuberculosis. Methods Case-control study was conducted. PCR-RFLP technique was used to detect the C/T polymorphism in VDR gene. Information on related factors of tuberculosis was collected using a pre-tested questionnaire. Univariate and multivariate logistic analyses were conducted with SPSS software package. Results A sample of 76 cases and 171 controls was studied. The genotype frequencies of VDR-FF, VDR-Ff and VDR-ff were 38.2 %, 44.7 %, 17.1 % and 52.6 %, 40.9 %, 6.4 % respectively. VDR-ff was significantly overrepresented in case group, the OR (95% CI ) was 3.668 ( 1.483- 9.071 ) when comparing with FF genotype. The significant association remained after adjusting BCG immunization and smoking, the OR (95% CI ) was 3.036 ( 1.117- 8.253 ). Conclusion The VDR-ff genotype might be associated with the susceptibility to pulmonary tuberculosis in Chinese Han population.