严重烧伤患者血清sFas和sFasL水平及其与感染的关系

Change of sFas and sFasL level and its relationship with infection in severe burned patients

目的 研究严重烧伤患者血清sFas和sFasL水平的变化规律 ,并探讨其与感染的关系。方法 采用ELISA法检测烧伤患者以及死亡患者血中sFas和sFasL水平 ,并与正常者比较。结果 (1 )被检者血清sFas和sFasL水平与对照者间差异有显著性意义 (P <0 0 5) ;伤后第 2周烧伤组与对照组的sFas水平间差别有显著性意义 (P <0 0 5) ,而第 3周时 ,sFas水平间差别无显著性意义 (P >0 0 5) ;伤后第 3周烧伤组与对照组的sFasL水平间差别有显著性意义 (P <0 0 5) ,而第 4周时 ,sFasL水平间差别无显著性意义 (P >0 0 5)。 (2 )患者血清sFasL水平与烧伤面积呈正相关 (r =0 2 2 1 ,P <0 0 1 )。 (3)感染组与非感染组的sFas和sFasL水平间差别均有显著性意义 (P <0 0 5)。 (4)死亡组与对照组的sFasL水平间差别有显著性意义 (P <0 0 1 )。结论 烧伤后sFas和sFasL水平直接影响机体免疫功能及凋亡的调控 。

Objective To study the change of sFas and sFasL and its relationship with infection in severe burned patients.Methods The levels of sFas and sFasL in the patients with severe burn and death were determined by ELISA method,and compared with the normal controls.Results (1)The levels of the serum sFas and sFasL in burned patients were significantly different than those in normal controls(P<0 05).The levels of sFas in burned patients were significantly higher than those in normal controls at the second week(P<0 05),and the sFas levels were the same outcome at the third week.There were no significant differences in the sFas levels at the third week between burn group and normal controls(P>0 05),and so was the sFasL at the fourth week(P>0 05).(2)The levels of sFasL were correlated with total burn surface area(r=0 221,P<0 01).(3)The sFas and sFasL levels in infection group were significantly higher than those in non_infection group(P<0 01～0 05).(4)The sFasL level in fatal cases was significantly increased in comparison with normal controls(P<0 01).Conclusion sFas and sFasL directly influence immune function and apoptosis regulation after burn,and they are closely correlated to the severity of burn and the infection.