骨胶原蛋白毒理安全性实验研究

The toxicological safety of bone collagen peptide

目的检测骨胶原蛋白的安全性。方法根据毒理学试验的基本原则,进行急性经口毒性试验、遗传毒性试验和大鼠30天喂养试验。结果 sD大鼠急性经口最大耐受剂量大于15g/kg.bw,属于无毒类。Ames试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验三项遗传毒性试验结果均为阴性。当最高剂量达到人体推荐摄入量的100倍时,骨胶原蛋白粉未引起大鼠整体健康状况、器官组织形态学和生理生化功能等各项重要指标的异常变化,初步估计最大无作用剂量大于10g/kg.bw(相当于人体推荐摄入量的60倍)。结论骨胶原蛋白是食用安全的。

Objective To assessment safety of bone collagen peptide.Methodes Fellowed the principle of toxicological test,the acute oral toxicity test,the genetic toxicity test and the 30 days feeding test in rats were used to judge the safety of bone collagen peptide.Result The acute oral maximum tolerated dose of SD rat was 15g/kg.bw,bone collagen peptide was nontoxic.The results of Ames test,Mouse bone marrow polychromatic erythrocyte micronucleus test and mouse spermatid malformation test were negative.Even the maximum dosage was hundredfold of body recommended dose,the general health condition,the organ histomorphology and the physiological and biochemical functions were normal.The maxial no-effect dose of bone collagen peptide was preliminary estimated was greater than 10g/kg.bw(60tomes body recommended dose) Conclusions Bone collagen peptide was food safty.