黑粒小麦漯珍1号的C-分带及GISH和PAGE分析

C-banding, GISH and PAGE analysis on Black Kernel Wheat Luozhen 1

为了解黑粒小麦漯珍1号的染色体构成、蛋白组分以及抗病性,利用染色体C-分带、基因组原位杂交(GISH)和PAGE分析对漯珍1号进行了系统鉴定。结果表明,漯珍1号含1对1BL/1RS易位染色体,另外,其7B染色体的C-分带带型也表现出与中国春有较大差异,而其余染色体则未见明显区别。其PMC M染色体平均构型为1.97(棒状二价体)+19.03(环状二价体),说明具有较好的细胞学稳定性。SDS-PAGE分析表明,其高分子量谷蛋白亚基(HMW-GS)组成为1、7+8和5+10;种子醇溶蛋白A-PAGE鉴定显示,除含有黑麦1RS GldB3编码的醇溶蛋白外,还有许多迁移率明显不同于中国春的多态性位点。抗病性初步鉴定结果表明,漯珍1号高感赤霉病和白粉病。

In China, at least 5 commercial black kernel wheat cultivars have been developed. However, their chromosomal and protein constitutions, as well as disease responses of these cultivars are less reported. In this paper, chromosomal configuration of black kernel wheat Luozhen 1 was characterized by chromosome C-banding, genomic in situ hybridization analysis. In addition to chromosome 7B differing from that of Chinese Spring, a pair of 1BL/1RS translocation was found in its chromosomal constitution. The mean chromosomal pairing configuration at PMC MⅠ of 1.97 +19.03 showed Luozhen 1 to be cytologically stable. SDS-PAGE analysis revealed that subunits of 1, 7+8 and 5+10 of HMW-Glu existed in Luozhen 1. The specific secalin of rye coded by 1RS GldB3 was also demonstrated, in addition, several polymorphic bands different from Chinese Spring after A-PAGE indicating more allelic variations in Luozhen1. Disease evaluation indicated that Luozhen 1 was highly susceptible to Fusarium head blight and powdery mildew.