摘要
RNA编辑是高等植物叶绿体基因转录后表达调控的一种重要方式。本研究利用生物信息学方法,预测出粗山羊草叶绿体基因组分布于15个蛋白编码基因的34个RNA编辑位点,均为C到U的转换,其中以ndhB最多,有9个编辑位点。通过与数据库中EST和高通量测序数据(SRA)进行比对分析,检测到分布于20个基因的29个编辑位点。综合上述研究结果,最终确定分布于9个基因的10个RNA编辑位点是真实存在的。分析这些位点RNA编辑现象对蛋白质结构的影响,发现ndhB编辑后β-折叠数增加,跨膜结构增加。这是首次有关粗山羊草叶绿体蛋白编码基因RNA编辑位点的报道。与禾本科水稻、玉米、大麦、黑麦和甘蔗的叶绿体RNA编辑位点进行比较,表明粗山羊草与黑麦、大麦的亲缘关系较近。本研究结果可为解析粗山羊草叶绿体基因的表达调控和探讨禾本科物种的起源进化提供理论依据。
RNA editing is one of the post-transcriptional regulation mechanisms of gene expression in the chloroplast of higher plants.By bioinformatics analysis,we predicted 34 RNA editing sites in 15 chloroplast coding genes of Aegilops tauschii,all of which are C to U conversion.Among them,ndhB is the most edited gene with 9 editing sites.Further more,29 editing sites were detected in 20 genes by blasting the public EST database and the available high-throughput sequence read archive(SRA)data.Finally,11 editing sites in 9 chloroplast genes were proved to be real.Protein structures analysis indicated that the ndhBediting leads to an increase in the sheets and transmembrane domains.Comparative analysis on 31 editing sites in 18 chloroplast coding genes with five Poaceae species suggested Aegilops tauschii had a close relationship with Lolium perenne and Hordeum vulgare.This study provides new information on studying chloroplast gene regulations as well as the origin and evolution of Poaceae species.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2014年第10期1341-1349,共9页
Journal of Triticeae Crops
基金
国家高技术研究发展计划(863计划)项目(2012AA10A308)
关键词
RNA编辑
粗山羊草
叶绿体
鉴定
分析
RNA editing
Aegilops tauschii
Chloroplast
Identification
Analysis
