TGF-β_1在ANA阳性自身免疫病中的诊断意义初探

Pilot Research of Diagnostic Effect of TGF-β_1 Detection in ANA Positive Autoimmune Diseases

目的 探讨转化生长因子β_1(TGF-β_1)在自身免疫性疾病诊断中的作用。方法 以临床诊断为自身免疫性疾病病例为研究对象,采用双抗体夹心ELISA法,测定了不同滴度ANA阳性及其它自身抗体SSA、SSB、Ul-RNP阳性患者血清中TGF-β_1的水平。结果 ANA 1:1 000阳性、ANA 1:10 000阳性患者的TGF-β_1水平均显著低于ANA 1:100阳性者(P<0.05);ANA 1:1 000和ANA 1:10 000伴有SSA、SSB、Ul-RNP(+)患者TGF-β_1水平显著低于单纯ANA 1:100、ANA 1:1 000、ANA 1:10000阳性(P<0.05)。结论 TGF-β_1水平的降低与高滴度ANA阳性及部分自身抗体阳性有关,提示TGF-β_1对自身免疫性疾病具有辅助诊断作用。

Objective To investigate the role of transforming growth factor β1 (TGF β1) in the diagnosis of autoimmune diseases. Methods Serum TGF β1 levels of the patients with different titer of ANA were detected utilizing double antibodies sandwich ELISA method. Results Serum TGF β1 levels in patients with serum ANA positive(1:1000 or 1 : 10 000) were significantly lowered compared with that in ANA positive(1 : 100) patients. TGF β levels in patients with serum ANA positive plus SSA, SSB and U1-RNP positive were significantly lowered than those with ANA positive alone (P< 0. 05). Conclusion Decrese.l serum TGF β1 levels are corrceted with high liters of ANA in patients with autoimmune diseases,indicating the possible diagnostic effect of HGF-β1 detection in autoimmune diseases.