微板核酸杂交-酶联显色检测性病沙眼衣原体DNA

Detection of Chlamydia Trachomatis DNA Using PCR-Microplate Hybridization-ELISA

目的 建立微板核酸杂交-ELISA方法,检测性病沙眼衣原体。方法 采用PCR、核酸杂交和ELISA三大生物技术相结合,建立微板核酸杂交ELISA法,并用此法分别同McCoy细胞培养和免疫学方法检测307份性传播疾病标本进行比较。结果 微板核酸杂交-ELISA法检测的阳性率为40.39％(122/307),高于细胞培养的9.77％(30/307)和免疫诊断的12.05％(37/307)。结论 微板核酸杂交-ELISA技术检测性病沙眼衣原体灵敏度高、特异性好、简便快速。因此,该方法具有很强的实用价值和发展前景。

Objective To detect Chlamydia Trachomatis DNA in the clinical patients using Microplate Hybridization-ELISA. Methods C . trachumatis DNA extracted from 307 urogemtal swab specimens with sexually transmitted diseases. Its PCR amplification products was hybridized to two specific probes to assay C. trachomatis DNA. The same specimens are detected by McCoy cell cul ure and type-specific monoclonal antibodies. Results The results of Microplate Hybridization-ELISA were compared with those McCoy cell culture and type-specific monoclonal antibodies test from 307 urogemtal swab specimens. The rates of prevalence of infection with C. trachomatis were 40. 39% ( 122/307) , 9. 77% (30/307 ) and 12. 05% (37/307). Conclusion Microplate Hybridization-ELISA is a more sensitive and specific method for clinic detection of C. trachmnatis.