人胎肝提取物对动物淋巴细胞增殖反应和肿瘤细胞体外生长的抑制作用

Inhibitory effect of human fetal-liver extract on the proliferation of animal lymphocytes and tumor cell growth in vitro

将人胎肝脏经过超速离心和硫酸铵沉淀而得到部分纯化的人胎肝提取物(HFLE),能抑制C_(57)BL/6小鼠和Wistar大鼠脾和胸腺细胞的增殖反应;并能抑制二株小鼠肿瘤细胞的体外生长;但对人三株肿瘤细胞无抑制作用。此种抑制作用没有种的特异性。HFLE对脾细胞增殖反应的抑制作用不是由于它在细胞外与ConA结合或除去ConA的作用,而可能是HFLE与脾细胞共同孵育时培养液中的精氨酸被HFLE所含精氨酸酶耗尽之故。HFLE对L929细胞生长的抑制作用可在RPMI 1640培养液中加入适量的L—精氨酸而被解除。

Human fetal-liver extract (HFLE) was partially purified by means of ultracentrifugation and ammonium sulfate precipitation from human fetal liver. HFLE can inhibite the proliferation of the spleen cells and the thymocytes of C_(57)BL/6 mice and Wistar rat, and the growth of 2 strains of murine tumor cells in vitro, but have no inhibitory effect on 3 strains of human tumor cells. This inhibitory activity is non-species specific. The inhibitory effect of HFLE on the cell proliferation is not due to the extracellular binding or the removal of Con A. It is likely that its inhibitory activity is due to the fact that the arginine in the culture medium is depleted by the argininase in HFLE when HFLE is incubated with spleen cells. The inhibitory effect of HFLE on the growth of L929 cells could be removed by adding appropriate amount of L-arginine to the RPMI-1640.