经鸭肝匀浆处理后中药抗HBsAg的作用

Effect of traditional Chinese medicinal herbs by the homogenate of the duck liver on HBsAg

用ELISA法研究了20种中草药煎剂经鸭肝匀浆作用后,抑制乙型肝炎病毒表面抗原(HBsAg)的效应。结果显示鸭肝匀浆作用前,虎杖,蚤休、木瓜、贯众、赤芍、大黄和黄连可明显抑制HBsAg的免疫活性。经37℃1h处理后,只有黄连能抑制HBsAg的免疫活性。其余受试药物的作用消失。结果提示机体生物代谢的酶类可能会影响一些中草药制剂破坏HBsAg的抗原性。

The effect of the decoctions of 20 traditional chinese medicinal herbs after duck liver homogenate on HBsAg was studied with ELISA. The result showed that before duck liver homogenate 7 medicinal herbs (Coptis chinensis franch, Polygonum cuspidatum, Paeonial lactiflora, Cyrtominum fortune, Rheum palmatum, Bail, Papaya, Zao shou) were able to inhibit the immune activity of HBsAg. After the treatment with duck liver homogenate (37℃ lh), only coptis chinensis franch was effective against the immune activity of HBsAg. The effects of the other medicinal herbs were not seen. This phenomenon shows that the enzymes of biometabolism may have influence on some traditional Chinese medicinal herbs to destroy the antigenicity of HBsAg.