黄芪对肠缺血再灌注肝损伤大鼠NO、MDA含量的影响

Influences of Astragalus Membranaceus Injections on Nitric Oxide and Malondialdehyde during the Hepatotoxicity Induced by Intestinal Ischemia-Reperfusion in Rats

目的观察黄芪对肠缺血再灌注所致肝损伤后大鼠一氧化氮 (NO)、丙二醛 (MDA)的影响。方法Wistar大鼠随机分为假手术组、肠缺血再灌注组、黄芪小剂量组、黄芪大剂量组。夹闭肠系膜上动脉1h ,再灌注 3h后分别测定各组血清、肝组织NO和MDA含量。结果大鼠肠缺血 1h、再灌注 3h ,血清、肝组织MDA、NO含量明显升高 ,不同剂量的黄芪注射液能使肠缺血再灌注肝损伤大鼠血清、肝组织的NO、MDA含量明显降低。结论黄芪通过抑制脂质过氧化损伤 ,降低NO及自由基损伤代谢产物MDA的含量 。

Objective To investigate the influences of Astragalus membranaceus injections on nitric oxide and malondialdehyde during the hepatotoxicity induced by intestinal ischemia reperfusion in rats.Methods Wistar rats were randomly divided into sham operated group,ischemia reperfusion(I/R) group,and pretreatment with astragalus membranaceus injections (3g/kg and 6g/kg )group.In the experiments,the root of superior mesenteric artery was blocked with non injury artery clap for 1 hour and reperfusion for 3 hours.Then the content of NO and MDA of serum and hepatic tissue were detected respectively.Results The content of NO and MDA of serum and hepatic increased in model group,but decreased in Astragalus membranaceus group.Conclusion Astragalus membranaceus injections can protect the liver of rats from injury induced by intestinal ischemia reperfusion by inhibiting the injury of lipid peroxidation and decreasing the contents of NO and MDA.