大鼠子宫内膜异位症模型的建立和评价

The Evaluation on Establishment of Endometriosis Model in Rats

目的建立大鼠子宫内膜异位症模型及实验方法。方法手术制备大鼠子宫内膜异位模型 ,观察形态学改变 ,配合子宫内膜抗体及雌二醇、孕酮水平的检测。结果大鼠子宫内膜异位模型组中抗子宫内膜抗体阳性率、血清雌二醇和孕酮均较假手术组及丹那唑组为高 (P <0 .0 1 )。病理学观察 ,模型组异位子宫的内膜腺体中腺腔扩大 ,腺上皮呈柱状或立方形。丹那唑治疗组的异位内膜腺体及腺上皮明显萎缩。结论手术制备大鼠子宫内膜异位症模型 ,操作简单 ,价格低廉 ,成功率高 。

Objective To establish the model of rats endometriosis and explore its evaluation methods.Methods The model of rats endometriosis was established by operation. Observe the morphologic modification, and detect the level of EMAB, estradiol and progesterone.Results Comparing with the sham operation group and Danazole group(P&lt;0.01),the positive rate of EMAB and the content of estradiol and progesterone in the model group were higher(P&lt;0.01).Pathological observation showed that in model group the ectopic endometrium gland lumems were dilatant and the glandular epithelium was cylindric or cubic, Whereas in the Danazol group the lumems of gland and glandular epithelium were obviously atrophic.Conclusion The operation to establish the endometriosis model in rats provides a simple,low priced and depentable method for the study of drug action.