室间隔缺损相关基因-BMPR下游基因的探讨

Identification of BMPR downstream genes related to ventricular septum defect

目的 :探索室间隔缺损相关基因 骨形态形成蛋白受体IA(BMPRIA ,又名ALK3)的下游心脏特异基因。方法 :使用PCR选择性cDNA减数法和基因芯片 (microarray)的方法筛选ALK3下游基因 ,比较两组mRNA。试验组的mRNA来自α MHCCre + / -、ALK3F/ -的 11.5d小鼠胚胎心脏。对照组的mRNA来自α MHCCre + / -、ALK3F/ +的 11.5d小鼠胚胎心脏。结果与结论 :在心脏特异的ALK3基因敲除下 ,血小板激活因子乙酰水解酶及转录因子Pax 8等基因的表达水平下降 ,其均是ALK3的重要下游基因 ,与室间隔缺损的形成有关 ;14 3 3蛋白 β亚型及蛋白酪氨酸激酶等基因的表达水平上调 。

Objective:To investigate the cardiac specific ALK3 downstream genes related to ventricular septum defect.Methods:The ALK3 downstream genes were screened using PCR select cDNA subtraction and microarray. Two populations of mRNA,one derived from the embryonic heart(11.5 days) of α MHC Cre+/-,ALK3 F/- mice,and the other derived from the α MHC Cre+/-,ALK3 F/+ mice,were compared. Results and Conclusion:It was found that the platelet activating factor acetylhydrolase and the box protein pax 8 gene et cetera were down regulated and may be important downstream genes in the BMP signaling pathway during interventricular septum development,the protein tyrosine kinase of focal adhesion kinase subfamily(PTK) and 14 3 3 protein beta subtype et cetera were up regulated and possibly are regulatory factors in this signaling pathway in the α MHC Cre+/-,ALK3 F/- mice.