乙型肝炎病毒前S1抗原检测及其临床意义

The detection and clinical significance of hepatitis B virus PreS1 antigen.

目的 为研究乙型肝炎病毒前S1抗原检测及其临床意义。 方法 用抗S和抗前S1单抗的双抗体夹心ELISA法对183例HBV感染者血清进行前S1抗原(Pre—S1)检测,其结果和HBVDNA聚合酶链反应(PCR)、乙型肝炎血清标志的检测结果进行比较。 结果前S1抗原在乙型肝炎病毒e抗原(HBeAg)阳性组的检出率为82.6％.显著高于HBeAg阴性组的检出率36.8％(P<0.01)。前S1抗原在HBV DNA阳性组的检出率为76.6％,显著高于HBV DNA阴性组的检出率42.4％(P<0.01)。前S1抗原和HBV DNA检测结果的符合率达70.5％,两者总体的检出率有关联(P<0.01),两者检出率的相关系数r=0.971(P<0.01)。 结论 前S1抗原可作为反映HBV复制与传染性的一个可靠标志。

Objective To study the detention and clinical significance of hepatitis B virus PreS1 antigen. Methods One hundred and eighty-three serum samples from HBV carriers were tested for the presence of PreSl antigen by ELISA, as well as HBV-DNA by PCR and serum markers by ELISA, then the results of the above tests were compared. Results The positive rate of the PreSl antigen in the HBeAg-positive group (82.6%) was significantly higher than that in the HBeAg-negative group(36.8%)(P<0.01). The positive rate of the PreSl antigen in the HBVDNA-positive group(76.6%) was significantly higher than that in the HBVDNA-negative group (42.4%)(P<0.01). The corresponding rate of PreSl antigen with HBV DNA tested was 70.5%, and there was correlation between total detection rate(P<0.01), the correlation coefficient was 0.971(P<0.01). Conclusion It is suggested that the PreSl antigen might be served as a reliable marker of hepatitis B vims replication and infection.