NF-κB诱导激酶基因突变对CD4^+CD25^+调节性T细胞产生的影响

Study of CD4^+CD25^+ regulatory T cells in NIK mutated mice

目的 :探讨NIK基因变异鼠—aly鼠自身免疫病的产生与CD4+ CD2 5 + 调节性T细胞的相关性。方法 :利用NIK基因自然突变鼠—aly aly鼠做为动物模型 ,aly +小鼠做为NIK基因正常对照鼠 ;CD4+ CD2 5 + T细胞亚群鉴定采用流式细胞分析仪 (FACS) ;用免疫组织化学方法观察胸腺结构。结果 :aly小鼠的CD4+ CD2 5 + CD8- 胸腺细胞数量和脾脏CD4+ CD2 5 + CD8- T淋巴细胞的数量明显低于aly +小鼠 ;aly小鼠胸腺髓质缺少UEA 1阳性细胞。结论 :NIK基因突变的aly小鼠CD4+ CD2 5 + 胸腺细胞和脾脏T淋巴细胞的数量明显降低可能是aly小鼠自身免疫病产生的原因 ;UEA 1阳性细胞很可能在CD4+ CD2 5 + 调节性T细胞选择中发挥作用。

Objective:To study the relationship betwen the mechanism of autoimmune disease and CD4+CD25+ T cell population in NIK mutated mice-aly mice.Methods:NIK mutated mice-aly/aly mice were used as model,aly/+mice as NIK normal control;cell populations were determined by FACS and the thymus structure were analyzed by immunohistochemistry.Results:The CD4+CD25+CD8- population were remarkably decreased in aly mice;and the UEA-1 positive cells were absent in aly mice.Conclusion:The autoimmune disease in aly mice might be the result of deceased the CD4+CD25+ population;the UEA-1 positive cells might play an important role in the development of CD4+CD25+ population. [