槐定对互隔交链孢霉霉菌毒素所致DNA损伤的抑制作用

Inhibitory Effects of Sophoridine on DNA Damage Induced by the Mycotoxins of Alternaria alternata

本文应用碱性洗脱荧光分析法探讨了槐定对互隔交链孢霉的两种毒素——交链孢酚单甲醚(AME)和交链孢酚(AOH)引起2BS细胞DNA单链断裂的抑制作用。实验结果表明,槐定能抑制AME和AOH引起的2BS细胞DNA单链断裂,加入不同浓度的槐定作用后洗脱9小时DNA在滤膜上的存留分数分别为:AME组,由对照组的0.205=0.02回升至0.57±0.02、0.61±0.05、0.74±0.03;AOH组,由对照组的0.11±0.01回升至0.45±0.04、0.58±0.03、0.85±0.06,实验组与对照组比较统计学处理均有显著差别(P<0.01)。

In this study, the alkaline elution and fluorometic DNA assay were used to explore the inhibitory effects of sophoridine on DNA damage induced by the alternariol monomethyl ether (AME) and alternariol (AOH) which were produced by alternaria alternata. The results showed that sophoridine was able to inhibit the DNA single-strand breaks induced by the AME and AOH in the cultured human fetal lung 2BS cells : (1)Under the sophoridine concentration of 12.5μg/ml,25μg/ml,50μg/ml,the fraction of DNA remaining on the filters in AME (50μg/ml) groups was 0.57±0. 02,0.61±0.05,0.74 ±0.03 respectively,while it was 0.20±0.02 in the control group (only with AME 50μg/ml). (2) Under the sophoridine concentration of 25μg/ml,50μg/ml, l00μg/ml,the fraction of DNA remaning on the filters in AOH (10μg/ml) groups was 0.45±0.04,0.58±0.03,0.85±0.06 respectively, while it was 0.11±0.01 in the control group (only with AOH 10μg/ml). The dosage-response relationships of both AME and AOH groups were clearly shown. The fraction of DNA remaining on the filters by test groups were significantly different from that by control gronps (P<0.01).