非肝炎口腔科患者血清及唾液中HGV和TTV感染率的调查

Infection rate investigation of HGV and TTV in sera and saliva from non-hepatitis patients with oral diseases

目的 检测杭州地区非肝炎口腔科患者血清和唾液标本中庚型肝炎病毒 (HGV)和输血传播病毒 (TTV)感染率 ,进一步了解HGV和TTV传播途径和感染的高危人群。方法 分别从上述患者血液和唾液标本中提取RNA和DNA ,采用逆转录 -套式聚合酶链反应 (RT -NestedPCR)和半套式聚合酶链反应 (Hemi-nestedPCR)分别检测HGV5’ -NCRRNA和TTVN2 2区DNA ,部分HGV和TTV扩增产物克隆后进行核苷酸序列测定。结果 2 2 6例血清标本中 ,仅HGV阳性 2 7例(11 9% )、仅TTV阳性 2 1例 (9 3% )、HGV和TTV均阳性 7例 (3 1% ) ;2 2 6例唾液标本中 ,仅HGV阳性 10例 (4 4 % )、仅TTV阳性 9例 (3 9% )、HGV和TTV均阳性 2例 (0 9% ) ;未见血清标本HGV和 /或TTV检测结果阴性而唾液标本阳性者。2例患者血清标本及唾液标本HGV和TTV均阳性的扩增产物分别与报道的HGV和TTV核苷酸序列比较 ,同源性为88 6 5 %～ 91 4 9%和 6 5 32 %～ 6 6 6 7% ,但各自的血清标本与唾液标本HGV和TTV扩增产物同源性分别高达 98 5 8%～99 2 9%和 98 6 5 %～ 98 2 0 %。结论 杭州地区非肝炎口腔科患者HGV或TTV感染率均较高 ,其中部分携带病毒的患者唾液中均可检出HGV或TTV ,提示HGV或TTV可能存在唾液传播途径 。

Aim To determine the frequencies of HGV and TTV infections in serum and saliva samples from non hepatitis patients with oral diseases in Hangzhou area,and to further understand the transmission pathways of HGV and TTV,and the populations at high risk of the infection.Methods RNA and DNA were respectively isolated from serum and saliva samples of the patients.HGV RNA at the 5' NCR and TTV DNA at the N22 region were detected by using RT nested PCR and Hemi nested PCR,respectively.Part of HGV and TTV amplification products were cloned and then sequenced.Results 27(11 9%),21(9 3%)and 7(3 1%) of the 226 serum samples were only positive for HGV,only positive for TTV and positive for both HGV and TTV,respectively.10(4 4%),9(3 9%)and 2(0 9%)of the 226 saliva samples were only positive for HGV,only positive for TTV and positive for both HGV and TTV,respectively.No saliva samples from the 226 patients were found to be HGV or TTV detectable while their serum samples were negative for HGV or TTV.Homologies of the nucleotide sequences of HGV and TTV amplification products from the serum and saliva samples of the two patients compared with the reported corresponding nucleotide sequences were 88 65%～91 49% and 65 32%～66 67%,respectively.In comparison with the nucleotide sequences of amplification products between serum and from saliva sample from any one of the two patients,the homologies were 98 58% and 99 29% for HGV,and were 98 65%and 98 20% for TTV,respectively.Conclusion Relatively high infection rates of HGV and/or TTV in the non hepatitis patients with oral diseases in Hangzhou area are demonstrated.Parts of the carries are HGV and/or TTV positive in their saliva.The results of this study indicate that by way of saliva,HGV and TTV may be transmitted among individuals,and dentists may be one of populations with high risk for HGV and/or TTV infection.