cagA(+)Hp圆球体实验动物模型的建立

Establishment of laboratory animal model of cagA(+) coccoid Helicobacter pylori

目的 建立Hp圆球体动物模型。 方法 通过去营养法来培养cagA(+)Hp圆球体 ,定期用MTT和SCC方法来检测细菌的代谢活性 ,用吉姆萨染色和电镜负染色来观察细菌在不同时期的形态和变化。在圆球体培养的第 7周 ,进行幽门螺杆菌及其圆球体经口感染裸鼠的实验。结果 通过细菌学、尿素酶实验、16SRNA、cagA(+)PCR检测和电镜负染色等方法确定从已喂服幽门螺杆菌及其圆球体的裸鼠胃里分离到的细菌克隆 ,经证实为cagA(+)Hp。 结论 首次在裸鼠上证实了Hp圆球体是Hp“粪—口”传播链上不可缺少的一环。

Aim To establish animal model infected with coccoid Hp Methods cagA(+) coccoid Hp was cultured and formed through nutrition deprivation,its metabolism was studied regularly with the methods of SCC and MTT the forms of different time was observed by Giemsa staining and TEM Experiment of infecting nude mice was done with cagA(+) coccoid Hp cultured as mentioned above for 7 weeks and with nomal cagA(+)Hp Result Bacteria isolated from nude mice infected by Hp or its coccoid form were comfirmed to contain cagA(+)Hp with assays of PCR,TEM and Urease Conclusion The results indicate that the coccoid form is a necessary link of Hp's 'stool-mouth' infection in nude mice