泡状棘球蚴病宿主淋巴细胞的变化及意义

Observation of lymphocyte from hosts with alveolar echinococcosis

目的 为探讨泡状棘球蚴病宿主体内淋巴细胞在免疫调节和发病中的作用。方法 对泡球蚴感染BALB/c小鼠观察至 2 5周 ,在不同时间取脾制备细胞悬液 ,检测CD+ 4 ,CD+ 8细胞数量。对 2 5例泡球蚴病患者和 18例健康人群 ,用FCM分析了CD+ 3 ,CD+ 4 ,CD+ 8,CD+ 19,CD+ 3 8,CD+ 56和HLA -DR+ 细胞的变化。结果 泡球蚴感染BALB/c小鼠后 ,1～ 8周以CD+ 4 细胞为主 ,随后CD+ 4 细胞减少 ,CD+ 8细胞增加 ,2 0周后改变显著 (P <0 0 5 ) ,CD+ 4 /CD+ 8比值迅速倒置。泡状棘球蚴病患者CD+ 3细胞未发生改变 ,CD+ 4 细胞较正常对照组下降 (P <0 0 5 ) ,CD+ 8细胞上升 (P <0 0 5 ) ,使CD+ 4 /CD+ 8比值降低 (P <0 0 5 )。CD+ 56细胞较正常对照组显著性降低 (P <0 0 1) ,CD+ 19,CD+ 3 8和HLA -DR+ 细胞未发生改变 (P >0 0 5 )。结论 泡球蚴感染小鼠前 8周 ,以CD+ 4 细胞反应为主 ,具有保护性免疫。感染后期逐渐以CD+ 8细胞为主 ,使机体呈免疫抑制状态 ,有利于泡球蚴生存。泡状棘球蚴病患者机体呈免疫抑制状态 。

Aim To explore the immunological regulation mechanism of host infected with alveolar echinococcus Methods The course of the infection was followed for 25 weeks,spleen cell from BALB/c mice infected with AE,CD + 4 and CD + 8 levels were determined by flow cytometry Expression of CD + 3,CD + 4,CD + 8,CD + 19 ,CD + 38 ,CD + 56 ,HLA DR + from 25 cases with AE was analyzed by flow cytometry Results 1-8 weeks of post infection,the CD + 4 cell showed a increase,and then decrease,which the CD + 8 cell gradually increased throughout the period after BALB/c mice was infected with AE There was a significant drop down that CD + 4/CD + 8 ratio since 20 weeks of post infection(P<0 05) CD + 4cells from patients with AE was significantly lower than that of control group(P<0 05),CD + 8 cells was significantly higher than that of control group(P<0 05) CD 56 +cells and CD + 4/CD + 8 ratio were remarkably decrease as compared with the controls(P<0 05),Conclusion CD 4 is mainly response in the early stage of infection,CD 4 response is replaced by CD 8 response in later period of infection AE patients exhibit a immune inhibition response,the immune inhibition response is very useful to patients with chronic alveolar echinococcus infection