圆弧青霉代谢物的诱变作用

Mutagenicity of the Metabolites Produced by Penicillium Cyclopium Westling

从食管癌高发区林县农户粮食中分离出19株圆弧青霉,随机选4株(45A_2,7B_3,155D_1,26B_1),在以甘露醇为碳源的Raulin-Thom培养基中培养。氯仿提取其代谢物,应用CHO(Chinese hamster ovary)细胞SCE(Sister Chromatid Exchange)法检测了它们的诱变作用,结果发现:45A_2加S_9后诱发SCE频率显著升高;7B_3,155D_1在不加S_9情况下诱发SCE频率显著升高;26B_1在加与不加S_9情况下均为阳性,但以不加S_9时作用较强,所测试的4株圆弧青霉代谢物均有不同程度的损伤细胞DNA作用,与食管癌的发生可能有一定关系。

Nineteen strains of Penicillium cyclopium westling(P. cyclopium)were isolated from the cereals of Linxian County,the high incident area of esophageal cancer. 4 strains were chosen randomly(45A_2, 7B_3, 155D_1,26B_1). They were cultured in Raulin-Thom medium for two weeks at the temperature of 25℃. The metabolites were extracted with CH_3Cl. The mutagenicity of the metabolites was tested with the method of Sister Chromatid Exchange(SCE). 45A_2 was positive in SCE test with S_9. The metabolites of 7B_3,155d_1 were positive without S_9. but they were negative with S_9. The metabolites of 26B_1 were positive with or without S_9. 4 strains of P·cyclopium could induce the damage of DNA to a certain extent. We suggest that there be some corelation between esophageal cancer and highly cereal-contaminated P·cyclopium.