人胃癌细胞系(BGC-823)的细胞遗传学研究

Cytogenetic Studies on BGC-823 Human Gastric Cancer Cell Line

本研究以人胃癌(BGC-823)细胞系为材料,自建系后40～60代进行了细胞遗传学研究。先后共计数600个分裂中期细胞的染色体数,并对其中的30个分裂相作了胰酶消化和G-分带。核型分析结果表明:染色体众数为亚三倍体,发现了规律性出现的标记染色体和Ⅰ号染色体长臂的部分缺失,部分核型出现双微小体。带型分析表明。Ⅰ号标记染色体为染色体11,12间的易位,t(11,12)(11pter→11q23::12q13→12qter).12号染色体的断裂点和染色体的脆性部位位于同一染色体区带(12q13)。这将有助于阐明此系的癌基因ras与标记染色体的关系,进而了解癌变过程中癌基因的作用。

Cytogenetic studies on human gastric cancer cell line,BGC-823,of the 40～60th passages,were performed. The chromosomes in 600 cell were enumerated and thirty metaphases with suitable chromosome length and good G-banding pattern were subjected to karyological analysis. The results demonstrated that the chromosome modal number appeared to be subtriploid, and that the marker chromosomes as well as deletion of distal end of chromosome 1 short arms(1p)were observed with a very high incidence. The banding pattern analysis showed that the marker chromosome 1 was a larger submidcentric chromosome and derived from translocation of chromosome 11 and 12,t(11, 12)(11pter→11q23 :: 12q13→12qter). The breakage site of chromosome 11 was just located at the constitutive fragile site(11q13). The results suggest that there may be a relationship between ras oncogene and the marker chromosome in the development and pathogenesis of cancer.