超广谱β-内酰胺酶快速检测方法研究

Extended-Spectrum β-lactamases(ESBLs) Rapid Detection

目的 寻找一种快速、准确、实用、价廉的ESBLs检测法。方法 增加培养基的营养 ,促进细菌繁殖 ,提高菌悬液浓度 ,利用TTC作为显色剂配制出新型的培养基 (简称TTC法 ) ,并与NCCLS推荐使用的ESBLs检测法 -标准纸片扩散确认法相比较。结果 两法符合率均达 98%以上 ,而TTC法在时间上缩短了 10h左右 ,仅需 6～ 8h。结论 本法快速、准确、实用、价廉 ,极适用于各大、小医院临床微生物实验室。

Objective To find a rapid, accurate, practical and economical ESBLs detection method Method To increase nutrition in the culture medium, facilitate bacterial growth, increase concentration of the suspension and use TTC as developer for the preparation of new culture medium (abbrev TTC method) and compared with results detected by the NCCLS recommended ESBLs test-the standard disc diffusion confirmatory test. Result The coincidence rate of the two methods reached 98% and above while the TTC method shortening the time by about 10 hours which took only 6～8hours to complete. Conclusion Fast, accurate, practical and less expensive, the method is adequate for clinical microbe laboratories of both large and small hospitals.