胃癌细胞的凋亡与p53蛋白异常表达细胞增殖抗原关系的研究

Study on the Correlation Apoptosis with Abnormal Expression of p53 Protein,PCNA in Gastric Carcinoma

目的 :探讨胃癌细胞的凋亡与p5 3蛋白的异常表达 ,细胞增殖抗原 (PCNA)之间的相互关系。方法 :应用DNA末端转移酶介导的Bio dUTP原位末端标记技术 (TUNEL)和免疫组织化学技术对 6 2例胃癌病例进行研究。结果 :p5 3蛋白的异常表达为 5 8.1%( 36 6 2 ) ,凋亡指数 (AI)阳性组为 2 .1%～ 7.4%,平均 ( 3 .6± 0 .92 ) %,阴性组为 7.6 %～ 18.8%,平均 ( 13 .6± 2 .4) %,P <0 .0 1;PCNA的阳性率为 6 7.8%( 42 6 2 ) ,AI阳性组为 1.8%～ 6 .4%,平均 ( 3.2± 0 .78) %,阴性组为 8.2 %～19 .6 %,平均 ( 14.8± 2 .7) %,P <0 .0 1。结论 :胃癌细胞的凋亡与 p5 3蛋白的异常表达和PCNA关系密切。

Objective:To explore the correlation apoptosis with abnormal expression of p53 protein, proliferating cell antigen (PCNA) in gastric carcinoma.Methods: The expression of p53 protein,PCNA and apoptosis were detected by immunohistochemical and the terminal deoxynucleotidy transeraes-mediated-dUTP-biotin nick end labeling(TUNEL) in 62 specimens.Results:The rate of abnormal expression of p53 protein was 58.1%(36/62),apoptosis index(AI) was 2.1 to 7.4(mean, 3.6±0.92) in positive group and 7.6 to 18.8 (mean, 13.6±2.4) in negative group (P<0.01). The rate of PCNA was 67.8%(42/62), AI was 1.8 to 6.4 (mean,3.2±0.78) in positive group and 8.2 to 19.6 (mean,14.8±2.7) in negative group (P<0.01). Conclusion:Abnormal expressions of p53 protein and PCNA were associated with apoptosis in gastric carcinoma.