酶联免疫吸附法检测类风湿关节炎患者部分促炎因子水平的变化及其意义

Detection of TNF-α, interleukin-6 and interleukin-17 level in rheumatoid arthritis patients using enzyme linked immuno-sorbent assay (ELISA)

目的:探讨采用酶联免疫吸附法(ELISA)检测类风湿关节炎(RA)患者肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-17(IL-17)表达水平的变化。方法:应用酶联免疫吸附法检测100例健康体检者和100例RA患者的TNF-α、IL-6及IL-17的表达水平,同时采用全自动生化仪检测类风湿因子(RF),用血球计数仪测定白细胞计数(WBC)、红细胞计数(RBC)、血小板计数(PLT)和血沉(ESR)等实验室指标。结果:RA患者组的TNF-α、IL-6及IL-17的表达水平以及RF,PLT和ESR的水平均高于健康对照组(P<0.05)。结论:TNF-α、IL-6及IL-17与RA免疫炎症有关,ELISA法可有效检测上述炎症因子的转录水平,对RA有辅助诊断作用。

Objective To study TNF-α,interleukin-6 and interleukin-17 level in rheumatoid arthritis(RA) patients using enzyme linked immuno-sorbent assay(ELISA).Methods TNF-α,interleukin-6 and interleukin-17 were detected in 100 patients with RA and 50 healthy controls by using ELISA.RF,WBC,RBC,PLT and ESR were examined respectively.Results The levels of TNF-α,interleukin-6,interleukin-17,RF,PLT and ESR in RA patients were significantly higher than that of healthy controls(P <0.05).Conclusion TNF-α,interleukin-6 and interleukin-17 level are closely associated with inflammation and disease activity in RA,while ELISA can be used as a aided diagnosis method for RA.