摘要
目的:探讨采用酶联免疫吸附法(ELISA)检测类风湿关节炎(RA)患者肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-17(IL-17)表达水平的变化。方法:应用酶联免疫吸附法检测100例健康体检者和100例RA患者的TNF-α、IL-6及IL-17的表达水平,同时采用全自动生化仪检测类风湿因子(RF),用血球计数仪测定白细胞计数(WBC)、红细胞计数(RBC)、血小板计数(PLT)和血沉(ESR)等实验室指标。结果:RA患者组的TNF-α、IL-6及IL-17的表达水平以及RF,PLT和ESR的水平均高于健康对照组(P<0.05)。结论:TNF-α、IL-6及IL-17与RA免疫炎症有关,ELISA法可有效检测上述炎症因子的转录水平,对RA有辅助诊断作用。
Objective To study TNF-α,interleukin-6 and interleukin-17 level in rheumatoid arthritis(RA) patients using enzyme linked immuno-sorbent assay(ELISA).Methods TNF-α,interleukin-6 and interleukin-17 were detected in 100 patients with RA and 50 healthy controls by using ELISA.RF,WBC,RBC,PLT and ESR were examined respectively.Results The levels of TNF-α,interleukin-6,interleukin-17,RF,PLT and ESR in RA patients were significantly higher than that of healthy controls(P <0.05).Conclusion TNF-α,interleukin-6 and interleukin-17 level are closely associated with inflammation and disease activity in RA,while ELISA can be used as a aided diagnosis method for RA.
出处
《中国美容医学》
CAS
2012年第12X期165-166,共2页
Chinese Journal of Aesthetic Medicine
基金
国家自然科学基金(31071216)
江苏省自然科学基金(BK2010306)
扬州大学科技创新培育基金(2010CXJ076)资助