1种新来源的内皮祖细胞提取分离方法

A new method for extraction and isolation of endothelial progenitor cells

目的探索1种新来源内皮祖细胞(endothelial progenitor cells,EPCs)的提取分离方法,以增加EPCs细胞数量、提高增殖活性。方法拟从胎鼠肺提取分离培养EPCs,分别从形态学、表面标志物、吞噬功能和体外管腔形成等方面验证所提取细胞为EPCs。同时将其与骨髓中提取的内皮祖细胞的细胞增殖活力进行比较。结果从胎鼠肺提取分离培养的细胞,其形态特征与EPCs一致,细胞表达CD31、CD34、CD133、VEGFR2等EPCs特异标志物,可同时吞噬Dil-ac-LDL和FITC-UEA-1;体外可形成管腔样结构,功能上符合EPCs的表现。胎鼠肺提取的细胞增殖活力明显高于传统从骨髓提取的EPCs。结论胎鼠肺中可提取出数量和质量较好的EPCs,为进一步实验提供可靠的细胞来源。

To explore a new method for the extraction and isolation of endothelial progenitor cells(EPCs) and increase the number of EPCs and the proliferative activity, we isolated EPCs from fetal rat lung and verified these cells with morphology, cell surface markers, phagocytosis and blood vessels formation. The proliferation activity of the isolated EPCs was compared with that of EPCs extracted from bone marrow. the cells isolated from fetal rat lung demonstrated the same morphological characteristics with EPCs, and expressed the specific markers of EPCs, such as CD31, CD34, CD133, VEGFR2 and so on. Also, these cells could engulf Dil-ac-LDL and FITC-UEA-1 and form lumen structure in vitro. All in a word, these cells extracted from fetal rat lung were authenticated to be EPCs.The proliferative activity of EPCs extracted from fetal rat lung was significantly higher than that from bone marrow.So it is feasible to extract EPCs from fetal rat lung, and to provide a reliable cell source for further experiments.