B7-H6 mRNA在结肠癌中的表达和临床意义

The expression and clinical significance of B7-H6 mRNA in colon cancer

目的探讨B7-H6 mRNA在结肠癌中的表达水平和临床意义。方法以65例结肠癌组织和15例癌旁组织为研究对象,使用q PCR检测B7-H6 mRNA表达水平,并结合临床资料,利用卡方检验分析B7-H6 mRNA表达水平与病理参数的相关性。采用Kaplan-Meier法(生存期分析)比较不同B7-H6 mRNA表达水平和患者预后的关系。结果通过确切概率法发现,不同B7-H6 mRNA表达水平的癌组织具有不同的肿瘤浸润程度,差异具有统计学意义(P=0.042),B7-H6 mRNA高表达组更倾向于外膜外浸润,而低表达组则倾向于深肌层浸润。而不同的B7-H6 mRNA表达水平与其他的病理参数(性别、年龄、肿瘤外形、病理类型、N分期以及临床分期)指标之间则差异无统计学意义(P值均>0.05)。生存期分析发现,与B7-H6 mRNA低表达组相比,B7-H6 mRNA高表达组结肠癌患者预后生存期显著降低,差异具有统计学意义(P=0.008)。结论高表达B7-H6 mRNA的患者的肿瘤更易于浸润到外膜外并累及其他组织器官,且高表达水平的患者更倾向于预后不良。

B7-H6 is a newly discovered member of the B7 family.The expression and clinical significance of B7-H6 mRNA in cancer tissues remains unclear.In this study,we intend to investigate the expression of B7-H6 mRNA in colon cancer by qPCR,and the correlation between the expression level of B7H6 and pathological parameters was analyzed by chi-square test combined with clinical data.Kaplan-Meier method(survival analysis)was used to compare the prognosis of patients with different levels of B7-H6 expression.The exact probability method showed that the cancer tissues with different B7-H6 expression levels had different degrees of invasion(P=0.042).The high expression group of B7-H6 gene tended to infiltrate the outer membrane,while the low expression group tended to infiltrate the deep muscle.However,there was no significant difference in the expression level of B7-H6 gene between different pathological parameters(sex,age,tumor shape,pathological type,N stage and clinical stage)(P>0.05).Survival analysis showed that the prognostic survival time of colon cancer patients with high expression of B7-H6 gene was significantly lower than those with low expression of B7-H6 gene(P=0.008).In conclusion,the tumors of patients with high expression of B7-H6 are more likely to invade the outer membrane and involve other tissues and organs,and patients with high expression of B7-H6 are more likely to have poor prognosis.