ARDS时HO抗氧化作用机制初探

The antioxidation of HO in ARDS induced by chemicals

目的 探讨血红素氧合酶 (HO)抗氧化性对急性呼吸窘迫综合征 (ARDS)的保护作用及其机制。方法 采用电子自旋共振 (ESR)技术直接测量活性氧 (ROS) ,并结合化学发光法和脂质过氧化产物测定等技术来研究ARDS发生发展过程中HO对自由基的影响。结果 ESR结果显示 ,大鼠注射OA后 10minROS含量即升高 ,30min达到高峰 ,以后逐渐下降 ;HO组未见ROS显著增加 ;而化学发光法定量检测和SOD检测结果与ESR波谱显示的结果完全一致 ;MDA检测结果则提示Hb诱导HO有一定的副作用。结论 ROS可能是ARDS启动的主要病因 ;

Objective To investigate the antioxidation of heme oxygenase(HO)in acute respiratory distress syndrome(ARDS)induced by injection of oleic acid.Method Reactive oxygen species(ROS)was measured by electron spin resonance(ESR)direct-ly,chemical luminescence,MDA and SOD were used to investigate the effect of HO on free radicals in ARDS.Result The spectrum of ESR showed that the concentration of ROS increased at10minutes and got summit at30minutes after injection of oleic acid(OA),then dropped gradually,whereas it didn t increase greatly in HO-treated groups,moreover,the results of quantified measure by chemi-cal luminescence and SOD assay corresponded with the tendency exhibited in ESR measurement,whereas MDA assay indicated that in-duction of HO by hemoglobin had some side effect.Conclusion The results demonstrated that ROS may be a key factor in the starting phase of ARDS.HO has definite ability of antioxidation.